WVU :: Health Sciences Center :: Research and Graduate Education

Office of Research and Graduate Education

Links

Phone:

304.293.7206 (Research)

304.293.7116 (Education)

P.O. Box 9104

Morgantown, WV 26506

Flow Cytometry Core Facility:

Techinical Director: Kathleen Brundage, Ph.D. (View Profile)
Location: Room 2160 Health Sciences Center North
(across from 2nd floor entrance to the Learning Center)
Contact Info: PO Box 9177
Morgantown, West Virginia 26506
Phone: (304)293-6273
Fax: (304)293-7823
Email: flowcore@hsc.wvu.edu

Services:

Eukaryotic Cell Analysis and Sorting:

Extracellular and intracellular antigen expression and quantitation
Antibody labeling
Transfected cells (GFP and YFP)
Cell function
Proliferation
Viability
Apoptosis
Necrosis
Cell cycle analysis
Functional assays
Mitochondria activity
Cell signaling/signal transduction
Cytokine production and quantitation

Prokaryotic Cell Analysis and Sorting:

Phenotyping
Quantitation
Viability
Enzymatic activity
Sorting

WVU Flow Cytometry Core Fee Schedule as of July 2011:

	Analysis By Users	Analysis By Facility	Sorting
WVU User	$33/HR	$50/HR	$74/HR
Other Users	$48.51/HR	$73.50/HR	$108.78/HR

* Sorting - Every 10th Hour is free

FACSCalibur:

Two lasers:
- 488 nm argon laser
- 633 nm red diode laser
Seven parameter analysis:
- Forward Scatter
- Side Scatter
- 4 different fluorochromes

User training is available

FACSAria:

FACSAria Sorter ( acquired 2006)
Three lasers:
- 488 nm Sapphire solid state laser
- 633 nm HeNe laser
- 407 nm Violet solid state laser
Fifteen parameter analysis:
- Forward Scatter
- Side Scatter
- 13 different fluorochromes
Sorting:
- separation into 2 – 4 populations
- sort into tubes or plates

AutoMacs Sorter:

AutoMacs Magnetic Sorter (2002)
Magnetic bead separation

Quick efficient single marker sorting based on cell surface phenotype

Upcoming Events: 2012 E.J. Van Liere Memorial Convocation
and HSC Research Day

Ph.D. Training Programs: Biomedical Sciences; Public Health Sciences; M.D./Ph.D. Medical Scientist; Health Outcomes Research; Nursing Research

Announcements!: Events and Seminars around the HSC.; Meet the new Vice President of Research

Contact Us: Research: 304-293-7206; Education: 304-293-7116; P.O. Box 9104; Morgantown, WV 26506; Webmaster