S. Jamal Mustafa, Ph.D.
	Assistant Vice President for Research Wyeth Research Scholar Professor of Physiology and Pharmacology and Basic Pharmaceutical Sciences Graduate Training: Locknow University, India Fellowship: C.S.I.R., New Delhi and the University of Virginia
	Office: 224-BBRF Lab: 270-BBRF PO Box 9104 Morgantown, WV 26506	Email: smustafa@hsc.wvu.edu Phone: 304-293-5830 Fax: 304-293-7038

Research Interests:

Overview

This laboratory is interested in understanding the cellular and molecular mechanism(s) of blood flow regulation to the heart. The major emphasis is on the role of adenosine and its nucleotides in the regulation of vascular tone and its signaling (such as calcium, role of cytochrome 450 system, G proteins, protein kinase, phosphoinositides, arachidonic acid pathways, cAMP, cGMP, NO, etc.) in relation to endothelium and vascular smooth muscle cells. Currently, we are using all four-adenosine receptor knockouts to address this topic. Also, we have isolated both the coronary smooth muscle and endothelial cells from the adenosine receptor knockout mice for our signaling work. Recently, we are now using models of coronary disease (APOE knockout with high fat diet) to address the signaling of adenosine receptors in vascular and heart tissues. Our projects involve physiology, pharmacology, immunology, molecular biology and biochemistry.

The second area of major interest of this laboratory is to define the role of adenosine in asthma in relation to airway hyperresponsiveness, inflammation and oxidative stress. We have identified the receptor subtype involved in airway hyperresponsiveness using an allergic mouse model of asthma. We are now utilizing all four subtypes of adenosine receptor knockouts to further characterize the receptor involved in this model. Also, we are using different allergens to maximize the airway inflammation in these knockouts to identify the receptor subtype on the inflammatory cells and their mediators including TH1 and Th2 cytokines. We are also interested as to how in this model asthma leads to systemic inflammation and vascular reactivity. Finally, we are also investigating how obesity and coronary disease influences lung function and inflammation.

I am a PI on a NIH-HLBI entitled “ Research Training in Cardiovascular and Pulmonary Diseases ”.

Selected Publications:

1. Nadeem, A., Obiefuna, PCM., Wilson, CN and S. J. Mustafa. A Novel Oral A 1 Adenosine Receptor Antagonist (L-97-1) Versus an Oral Leukotriene Receptor Antagonist (Montelukast Sodium) on Allergic and Inflammatory Responses to House Dust Mite in a Rabbit Model of Asthma. Eur. J. Pharmacol. 551: 116-124, 2006.
2. Teng, B, Ansari, HR, Oldenburg PJ, Schnermann J, and S. J. Mustafa. Isolation and Characterization of Coronary Endothelial and Smooth Muscle Cells from A 1 Adenosine Receptor Knockout Mice. Am J Physiol Heart Circ Physiol. 290:H1713–H1720, 2006.
3. Barakat H, Lang D, Davis J, S. J. Mustafa and M. M. McConnaughey Differences in the Expression of the Adenosine A1 Receptor in Adipose Tissue of Obese Black and White Women. J Clin Endocrinol Metab. 91(5):1882-6, 2006.
4. Morrison RR, Teng B, Oldenburg PJ, Katwa LC, Schnermann J and S. J. Mustafa. Effects of Targeted Deletion of A 1 Adenosine Receptors on Post-Ischemic Cardiac Function and Expression of Adenosine Receptor Subtypes. Am J Physiol Heart Circ Physiol. 291(4):H1875-82, 2006 .
5. Tawfik HE, Teng B, Morrison RR, Schnermann J and S. J. Mustafa. Role of A 1 Adenosine Receptor in the Regulation of Coronary Flow. Am J Physiol Heart Circ Physiol. 291(1):H467-H472, 2006.
6. Ansari HR, Nadeem A, Talukder MAH, Sakhalkar S, S. J. Mustafa. Evidence for the Involvement of Nitric Oxide in A 2B receptor-Mediated Vasorelaxation of Mouse Aorta. Am J Physiol Heart Circ Physiol. 292(1):H719-25, 2007.
7. Nadeem A, Fan A, Ansari HR, Ledent C and S. J. Mustafa. Airway inflammation and hyperreponsiveness in A 2A adenosine receptor deficient allergic mice. Am J Physiol Lung Cell Mol Physiol. Feb 9, 2007.
8. Mustafa, S. J., Nadeem A, Fan M, Zeng D, Zhong H and Belardinelli L. Effect of a specific and selective A2B adenosine receptor antagonist on adenosine agonist, AMP and allergen-induced airway responsiveness and inflammation in a mouse. model of asthma . J Pharmacol Exp Ther. 320(3):1246-51, 2007.
9. Morrison, R. Ray; Tan, Xing Lin; Ledent, Catherine; Mustafa, S. J; Hofmann, Polly. Targeted Deletion of A2A Adenosine Receptors Attenuates the Protective Effects of Myocardial Postconditioning. Am J Physiol Heart Circ Physiol. 293(4):H2523-9, 2007.
10. Ansari HA, Nadeem A, Tilley SL, Mustafa SJ. Evidence for the involvement of COX-1/COX-2 in A₃ adenosine receptor-mediated contraction through endothelium in mouse aorta. Am J Physiol Heart Circ Physiol. 293(6):H3448-55, 2007.
11. Dovenia S. Ponnoth, Ahmed Nadeem and Mustafa SJ. Adenosine-mediated alteration of vascular reactivity and inflammation in a murine model of asthma. Am J Physiol Heart Circ Physiol. 294(5):H2158-65, 2008.
12. Li CH, Zhang Q, Teng B, Mustafa SJ, Huang JY, Yu HG. Src Tyrosine Kinase Alters Gating of Hyperpolarization-Activated HCN4 Pacemaker Channel through Tyr531. Am J Physiol Heart Circ Physiol 294(1):C355-62, 2008.
13. Teng B, Ledent C,  and Mustafa SJ. Up-regulation of A_2B Adenosine Receptor in A_2A Adenosine Receptor Knockout Mouse Coronary Artery. J. Mol. Cell. Cardiol. 44(5), 905-914: 2008.
14. Masood A, Nadeem A, Mustafa SJ, O'Donnell JM. Reversal of oxidative stress-induced anxiety by inhibition of phosphodiesterase-2 in mice. J Pharmacol Exp Ther. 326(2):369-79, 2008.
15. Nayeem MA, Falck, JR, Ledent C, Ponnoth DS, Ansari HR, Mustafa SJ. Enothelium dependent adenosine-mediated vascular responsesresponses in mouse aorta: Relationship between of A2A AR and CYP 450s. Am J Physiol Heart Circ Physiol. 295(5):H2068-78, 2008.
16. Nadeem A,Dovenia PS, Batchelor T, Dey R, Ledent C, Mustafa SJ. A2A AR deficiency results in decreased tracheal relaxation via NADPH oxidase pathway in an allergic mouse model of asthma.  J Pharmacol Exp Ther. 2009 Apr 24. [Epub ahead of print]
17. Nayeem MA, Ponnoth DS, Boegehold MA, Falck JR, Zeldin DC and Mustafa SJ. High salt diet enhances mouse aortic relaxation through adenosine A2A receptor via CYP epoxygenases. Am.J Physiol Regul.Integr.Comp Physiol. 296(3):R567-R574, 2009. PM:19109366
18. Ansari HR, Teng B, Nadeem A, Roush KP, Martin KH, Schnermann J, Mustafa SJ. A1 adenosine receptor-mediated protein kinase C signaling in mouse coronary artery smooth muscle cells. Am J Physiol Heart Circ Physiol. 2009 Jul 10. [Epub ahead of print]
19. Mustafa SJ, Ansari HR and Abebe W. P1 Purine Receptors in Current Protocols in Pharmacology. 2nd Edition, Pub. John Wiley & Sons, 2009 (In Press).
20. Wilson C, Nadeem A, Spina D, Brown R, Page CP and Mustafa SJ. Adenosine Receptors and Asthma. Adenosine Receptors in Health and Diseasse, Handbook of Experimental Pharmacology, Volume 193, Adenosine Receptors in Health and Diseasse, Editors, C. Wilson and S. J. Mustafa, Springer- Verlag GmbH, pages 329-62, (2009).
21. Mustafa SJ, Morrison RR, Teng B and Pelleg A. Adenosine’s Signal Transduction in the Heart Role in Coronary Hemodynamics and Cardiac Electrophysiology. Handbook of Experimental Pharmacology, Volume 193, Adenosine Receptors in Health and Diseasse, Editors, C. Wilson and S. J. Mustafa, Springer- Verlag GmbH, pages 161-88, (2009).

Lab Personnel: Bunyen Teng, DVM, Ph.D., - Research Assistant Professor Kevin Roush, - Research Specialist Dovenia Ponneth, Ph.D. - Postdoctoral Fellow Swati Kunduri - Ph.D. Student Maryam Sanjani Sharifi- Ph.D.Student Ernest J. Young- Ph.D.Student
	Bunyen Teng, Ernest Young, Maryam Sharifi-Sanyani. Jamal Mustafa, Kevin Rousch, Swati Kunduri and Dovenia Ponneth