The equipment that will be used for in vivo imaging is the Xenogen IVIS® Lumina Imaging System.  The components of this system include a cooled CCD camera, Imaging Chamber, acquisition computer with Living Image® software, and Integrated Gas Anesthesia system. 
CCD Camera:

• 13 x 13 mm square with 1024 x 1024 pixels 13 microns in width
• Back-thinned, back illuminated grade 1 CCD
• 16 bit digitizer
• Thermoelectrically (Peltier) cooled to -90°C

Imaging Chamber:

• Light-tight
• High light collection lens, f/0.95 – f/16
• 8 position emission filter wheel
• LED lamps for photographic images
• Heated stage to maintain optimum body temperature
• Motor controlled stage, filter wheel, lens position, and f-stop

Integrated Gas Anesthesia:

• Gas anesthesia (isoflurane) ports and 5 position manifold within imaging chamber

Living Image® Software:

• Photo brightness and color bar adjustment
• Image annotation tools
• Region of interest (ROI) measurements
• Absolute calibration of image intensity against NIST radiance standards
• Corrections for cosmic rays, dark charge, and flat field
• Printing, layout, and image export functions

IVIS System Control Panel:

• Controls for both fluorescence and bioluminescence imaging
• Manual and auto focus
• Setting of exposure time, binning, f/stop, and field view
• Fluorescence lamp and filter controls
• Acquisition setup of a single image or an image sequence