This Image Analysis Facility is available to the West Virginia University research community. The facility provides access to advanced biomedical imaging technology including state of the art light microscopy and quantitative software applications. The goal of this facility is to assist West Virginia University researchers in deriving quantitative data from a diverse range of image sources including electrophoresis, photomicrographs, transparencies, videotape, digital images, histological preparations and in vitro specimens. In addition, the facility assists the researcher to incorporate images and data collected for use in grant applications, publications and presentations. This facility has a Zeiss LSM 510 confocal microscope, an Optimas Image analysis system, MicroBrightfield stereo investigator and Neurolucida, Codonics 1600 Dye sublimation printer, Fujix pictography 3000 color printer, Focus imaging ImageCorder slide maker, flatbed scanner with transparency adapter, Optronics MagnaFire microscope digital camera.

The facility also has a live cell imaging system – He also has a Nikon E600 upright fluorescence microscope with a Spot R/T cooled CCD digital camera and associated computer software for image acquisition and analysis as well as a Nikon E200 inverted fluorescence microscope equipped with an ImageMaster-1700DDR digital imaging control module designed and dedicated for live cell imaging and analysis. Image capture and analysis will be obtained with the a Roper CoolSNAP-fx 12bit color CCD camera using the Metamorph Complete imaging software system driven by a custom built PC.

This unit is outfitted with a Peltzier heated stage, appropriate cubes and dichroics for FRET analysis and analysis for immunofluorescence at 4 separate emission spectra. This facility is available to users on a charge basis. Users also request support from grants for this facility. A full-time technician is on staff to assist users. Also available in the cancer center is a Zeiss Axiovert fluorescent microscope with Axiovision advanced softwared, Peltzier heated stage for time lapse and apotome, providing the equivalent of a confocal image acquisition and analysis system.

For more information vist the MIF website: http://anatomy.hsc.wvu.edu/mif

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