The Flow Cytometry Core Facility provides instrumentation and scientific support for cell analysis and cell sorting on a fee basis. The facility is equipped with two cytometers a Becton-Dickenson FACSCalibur and a FACSAria along with a Miltenyi Biotec AutoMACS magnetic bead cell sorting system. The FACSAria is a 15 parameter high speed bench top sorter capable of sorting cells into 4 separate populations. It has the capacity to sort into tubes or tissue culture plates and has both an aerosol management system for aseptic sorting and a temperature control option for maintaining the temperature of sorted samples. The FACSCalibur is equipped with an autoloader and has the capability of 4 color analysis. The Flow Cytometry Core has several software packages including CellQuest Pro, ModfitLT and BD Diva software for data acquisition and analysis. Services provided by this facility include analysis of cell surface phenotype, intracellular protein expression, cell cycle analysis, cytokine production, and cell sorting of eukaryotic cells. In addition, phenotypic analysis, quantitation and sorting of prokaryotic cells is available. In addition, Core staff is available for consultations on experimental design and data analysis.

For more information please visit this West Virginia University School of Medicine link: http://www.hsc.wvu.edu/som/micro/lab/flowlab.asp

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