Micromass Maldi and Finnegan LC-MS (ion trap) mass spectrometers were purchased using an NCRR equipment grant (S10-RR16792-01) and are available to perform high throughput and sensitive analysis of proteins. The proteomics core facility has 2-D gel separation apparatuses, DIGE technology, a Typhoon scanner to measure changes in fluorescence intensity, as well as Robotics integrated with a computer that permits an investigator to scan a 2-D gel, highlight a protein spot of interest and instruct the robotic arm to precisely pick and excise the protein of interest, transferring it to an elution chamber and then to a trypsin-digestion chamber. Technicians then take the digest and introduce it to the mass spectrometer for identification based on time of flight technology. The mass spectrometer then sends the sequence to the computer which matches it against known databases.

A Proteomics Facility was established using the CoBRE grant. 900 ft of lab space has been renovated for this facility, which exists on the second floor of the Health Sciences building at the School of Medicine. A core manager and a technician with mass spectrometry experience manage the facility.

To find out more about Proteomics at Mary Babb Randolph Cancer Center visit: http://www.hsc.wvu.edu/som/pathology/proteomics.asp

back to the Shared Resouces list (Cores, Core Facilities)