|
Stephen G. Graber, Ph.D. |
 Associate Professor
PhD: University
of Vermont
Postdoctoral Fellowship: University of Virginia
Joined the faculty:
WVU: 1993, Biochemistry: 2001
Affiliations:
Teaching: PCOL
743, PCOL 760, PCOL 761, MS1 PBL, BIOC 339, BMS 705,
CCMD 793L, PCOL 260
Room: 3122
Phone: (304) 293-2305
Fax: (304) 293-6846
Email:
sgraber@hsc.wvu.edu
|
|
|
|
Research Interests: |
|
|
|
The
laboratory is interested in contributing to the
understanding of the molecular mechanisms underlying G
protein mediated signal transduction.
Description of Research:
A striking feature of G protein coupled signaling
pathways is the large number of distinct subtypes
identified for each component. At the biochemical level
significant contributions in this area can be made by
developing the ability to employ purified components of
signal transduction pathways in reconstitution
paradigms. A baculovirus expression system has been used
to produce individual components of various signal
transduction pathways. The primary focus has been on the
potential role for specific combinations of G proteins
as determinants of selectivity in signaling pathways.
Among the issues being addressed are:
-
whether G protein heterotrimers are formed
selectively from distinct a and bg subunits;
-
whether specific a or bg subunits are determinants
of G protein coupling to specific receptors;
-
which specific domains on the subunit interact with
receptors;
-
whether the subunit composition of the G protein
coupled to a given receptor affects the ability of
the receptor to modulate the activity of its' target
effector.
Dr. Graber is a member of the Interdepartmental
Graduate Program in
Neuroscience.
|
|
|
|
References:
|
|
|
- Slessareva JE, Ma H, Depree KM, Flood LA, Cabrera-Vera TM, Hamm HE and Graber SG: "Closely related G protein coupled receptors use multiple and distinct domains on G protein α subunits for selective coupling" J. Biol. Chem. 278:50530 - 50536 (2003).
- Ma H., Peterson YK, Bernard ML, Lanier SM and Graber SG: "Influence of Cytosolic AGS3 on Receptor-G Protein Coupling" Biochemistry 42: 8085-8093 (2003).
- Slessareva JE and Graber SG: "Reconstitution Reveals Additional Roles for N- and C-Terminal Domains of G in Muscarinic Receptor Coupling" Biochemistry 42: 7552-7560 (2003).
- Cabrera-Vera TM, Thomas TO, Vanhauwe J, Depree KM, Graber SG and Hamm HE: "Dissecting receptor-G protein specificity using G alpha chimeras" Meth. Enzymology 344:69-81 (2002).
- Peterson YK, Hazard S, Graber SG and Lanier SM: "Identification of structural features in the GPR motif required for regulation of heterotrimeric G-proteins" J. Biol. Chem. 277:6767-6770 (2002).
- Cordeaux Y, Nickolls SA, Flood LA, Graber SG and Strange PG: "Influence of G-protein subtype on signaling through the D2long dopamine receptor" J. Biol. Chem. 276:28667-28675 (2001).
- Peterson YK, Bernard ML, Ma H, Hazard S, Graber SG and Lanier SM: "Stabilization of the GDP-bound Conformation of Gi a by a Peptide Derived from the G-protein Regulatory Motif of AGS3" J. Biol. Chem. 275: 33193-33196 (2000).
- Kazmi MA, Snyder LA, Cypess AM, Graber SG and Sakmar TP: "Selective Reconstitution of Human D4 Dopamine Receptor Variants with Gi Subtypes" Biochemistry 39: 3734-3744 (2000).
|
|
