Research Assistant Professor of Microbiology, Immunology & Cell Biology Ph.D., University of Pennsylvannia, 1993 2055 Health Science North (Office) (304) 293- 6273 (Office) 2160A Health Science North (Lab) Flow Cytometry Core Facility kbrundage@hsc.wvu.edu Curriculum Vitae

Academic Interests:

My research focuses on how chemical exposure affects the immune system. One of the chemicals we study is ethanol. For this project, an in vitro culturing system has been established in the laboratory to examine the effect of ethanol on the differentiation of human hematopoietic stem cells (CD34 + CD38 - ) into B cells. The long term goals of this project is to determine the mechanism by which ethanol affects B cell development and to develop an in vitro B cell differentiation system using human CD34 + CD38 - cord blood cells that can be used for screening chemicals for their immunotoxic potential as it relates to B cell development.

In our other studies, we are studying the effects that four herbicides bentazone, isoxaflutole, atrazine and propanil have on the immune system. For these projects, we have been investigating the effects that they have both individually and in combination on T cell function. Utilizing both primary cells and human T cell lines we have determined that one of the effects of chemical exposure to these herbicides is an inhibition of cytokine production by the T cells. Since cytokines are important mediators of immune responses any alteration in their production can affect the immune system's ability to respond to pathogens. The long term goal of these projects is to elucidate the mechanism involved in the immunosuppression.

Publications:

Brundage, Kathleen M., Mahaney, James E. and Barnett, John B., 2003. The amide class herbicide 3,4-dichloropropionanilide (DCPA) alters the mobility of hydrocarbon chains in T lymphocyte but not macrophage membrane, Journal of Toxicology and Environmental Health, Part A. 66: 1-13.

Brundage, K. M., Schafer, R. and Barnett, John B. 2004. Altered AP-1 (activating protein-1) activity and c-jun activation in T cells exposed to the amide class herbicide 3,4-dichloropropionanilide (DCPA). Toxicological Sciences. 79:98-105.

Brundage, Kathleen M. 2005. Signal transduction during lymphocyte activation. Encyclopedia Reference of Immunotoxicology. Springer-Verlag New York Inc. ed. Hans-Werner Vohr pp 586-590.

Pal, K., Kaetzel, C.S., Brundage, K.M., Cunningham, C.A., and Cuff, C.F. 2005. Regulation of polymeric immunoglobulin receptor expression by reovirus. Journal of General Virology 86: 2347.