kbrundage@hsc.wvu.edu

Technical Director:
Kathleen Brundage, Ph.D.

Location:
Room 2160 Health Sciences Center North
(across from 2nd floor entrance to the Learning Center)

Contact Information:
PO Box 9177
Morgantown, West Virginia 26506
Phone: (304)293-6273
Fax: (304)293-7823
email: flowcore@hsc.wvu.edu

Eukaryotic Cell Analysis and Sorting:

- Extracellular and intracellular antigen expression and quantitation
- Antibody labeling
- Transfected cells (GFP and YFP)
- Cell function
- Proliferation
- Viability
- Apoptosis
- Necrosis
- Cell cycle analysis
- Functional assays
- Mitochondria activity
- Cell signaling/signal transduction
- Cytokine production and quantitation

Prokaryotic Cell Analysis:

- Phenotyping
- Quantitation
- Viability
- Enzymatic activity
- Sorting

Two lasers:

    -488 nm argon laser
    -633 nm red diode laser

Seven parameter analysis:
    -Forward Scatter
    -Side Scatter
    -4 different fluorochromes

User training is available

FACSAria Sorter ( acquired 2006)

Three lasers:
    -488 nm Sapphire solid state laser
    -633 nm HeNe laser
    -407 nm Violet solid state laser

Fifteen parameter analysis:
    -Forward Scatter
    -Side Scatter
    -13 different fluorochromes

Sorting:
    -separation into 2 – 4 populations
    -sort into tubes or plates
    -Aseptic sorts
    -Temperature control of samples

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Magnetic bead separation

Quick efficient single marker sorting based on cell surface phenotype

AutoMacs Magnetic Sorter (2002)