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| Department of Physiology and Pharmacology Ping Lee Professor of Physiology Emeritus Ph.D., Duke University, 1964 Postdoctoral Fellowship, Duke University Phone: 304-293-1509 Email: plee@hsc.wvu.edu Research Interests Membrane transport during cell differentiation and maturation, and applications of x-ray microanalysis in biomedical sciences. Description of Research The focus of my research is on studying the transitory changes of membrane transport in maturing erythroblastic cells in the bone marrow and in differentiating human leukemic cells in culture. Immunochemical as well as molecular biological techniques are used to investigate the subunit and isoform changes of the membrane transporter, Na,K-ATPase. X-ray microanalysis is used in combination with cryosectioning technique to examine single cell elemental composition. Correlations between morphological changes and ion transport alterations of cells during erythropoiesis and myelocytic differentiation are investigated. The goal of this research is to understand the basic mechanisms involved in membrane transport maturation. Recent Publications Kirk R Gary, Michael Gates, Chun-Shia Chang, and Ping Lee. Distribution of cisplatin in bone marrow cell: x-ray imaging. Exptl Mol Path 63:33-40, 1996. Lee Ping, Michael Gates, Chun-Shia Chang, and R Gary Kirk. X-ray microanalysis and mapping: measuring uptake and intracellular distribution of labeled molecules. Ch. J Physiol 39(4):205-210, 1996. Kirk R Gary, Michael Gates, Aaron Provance, and Ping Lee. Quantitative x-ray images calculated on the basis of mass and volume. Microscopy and Microanalysis 3:512-519, 1997. Chang Chun-Shia, R Gary Kirk, and Ping Lee. Presence of immunoreactive a 3 isoform of Na,K-ATPase in mitochondria of kidney. Kidney International 54:457-463, 1998. Chang Chun-Shia, R Gary Kirk, and Ping Lee. Transient increase of a 3 isoform of Na,K-ATPase in erythroblastic cells. Histochem J 30:811-818, 1998. Kim H D, J T Turner, J E Burnett, N S Soo, B H Han, R W Lim, J W Bowen, R G Kirk, and P Lee. Furosemide stimulates K transport in HCD57 erythroid cells. J Memb Biol 175:235-244, 2000. |
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