In-vivo microscopy

Experimental setup for microscopic observation of the rat spinotrapezius muscle

 

 

Low-power view of the spinotrapezius muscle microcirculation as seen with in-vivo microscopy

 

 

Microvascular Endothelial Cell Isolation

General scheme for the isolation and culture of endothelial cells from precapillary microvessels in rat mesentery. The same general approach is used for the isolation and culture of postcapillary microvessels, except that iron particles are infused into the arterial network for the initial separation and removal of precapillary and capillary vessels.

 

 

Near-confluent monolayer of precapillary endothelial cells

Uptake of fluorescently-labelled acetylated low-density lipoprotein by precapillary endothelial cells