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Research and Graduate Education
Shared Research Facilities
Center for Advanced Imaging
Located in the Health Sciences Center, the Center for Advanced Imaging is a 12,000 square-foot research and clinical imaging facility. The Center currently operates a General Electric Advance PET scanner, a General Electric PETtrace cyclotron (with automated modules for the production of 18FDG and 11C-methyl-iodide), a General Electric 1.5T Signa LX MR scanner, and a 3T General Electric Research MRI System.
The Center for Advanced Imaging is currently undergoing a major expansion that emphasizes human brain mapping using functional MRI (fMRI). The first phase of this expansion includes the installation of the 3T MRI system, and the new facility houses approximately 8,000 square feet of renovated office and laboratory space.
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Clinical Trials Research Unit (CTRU)
Through a wide range of administrative and budgetary services, the CTRU provides clinical investigators and private industry with the services and expertise to conduct clinical trials and expand the growing body of biomedical knowledge. Clinical trials are conducted at the Mary Babb Randolph Cancer Center and the Robert C. Byrd Health Sciences Center.
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Image Analysis Facility
Operated by the Department of Neurobiology and Anatomy in the School of Medicine, the Image Analysis Facility serves the entire WVU research community, providing quantitative data from a range of image sources including electrophoresis, photomicrographs, transparencies, videotape, digital images, preparations, and in vitro specimens. In addition, the facility helps researchers incorporate images and data collected for use in grant applications, publications and presentations.
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Flow Cytometry Core
The Flow Cytometry Core Facility provides instrumentation and scientific support for cell analysis and cell sorting on a fee basis. The facility is equipped with two cytometers a Becton-Dickenson FACSCalibur and a FACSAria along with a Miltenyi Biotec AutoMACS magnetic bead cell sorting system. The FACSAria is a 15 parameter high speed bench top sorter capable of sorting cells into 4 separate populations. It has the capacity to sort into tubes or tissue culture plates and has both an aerosol management system for aseptic sorting and a temperature control option for maintaining the temperature of sorted samples. The FACSCalibur is equipped with an autoloader and has the capability of 4 color analysis. The Flow Cytometry Core has several software packages including CellQuest Pro, ModfitLT and BD Diva software for data acquisition and analysis. Services provided by this facility include analysis of cell surface phenotype, intracellular protein expression, cell cycle analysis, cytokine production, and cell sorting of eukaryotic cells. In addition, phenotypic analysis, quantitation and sorting of prokaryotic cells is available.
The Flow Cytometry Core Facility is located in room 2160 Health Sciences Center North.
The facility is managed by Dr. Kathleen Brundage, Ph.D. (kbrundage@hsc.wvu.edu) who oversees its day-to-day operations, operates the FACSAria for cell sorting, and provides necessary instrument and software training. In addition, she is available for consultations on experimental design and data analysis. To learn more about the facility contact Dr. Brundage at (304)293-6273.
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Laboratory Animal Resources
The primary WVU facility for maintaining animals for biomedical research and teaching is located at the Robert C. Byrd Health Sciences Center (HSC ). Designed to provide centralized procurement and care of animals, this facility consists of approximately 25,000 square feet of animal holding and support space.
The HSC facility can support a number of species, including rodents, rabbits, carnivores, small ruminants, swine, nonhuman primates, amphibians, reptiles, and fish. A surgical suite fully equipped for major survival surgeries is available, and additional special support units including a radiology suite and a Cesium 137 small animal irradiator.
Office of Laboratory Animal Resources (OLAR) personnel provide for husbandry and veterinary care of animals, research and teaching support, and investigator training. The Director of OLAR is a full-time attending veterinarian who is a board certified specialist in laboratory animal medicine (Diplomate, American College of Laboratory Animal Medicine). The OLAR animal facilities are also staffed with a registered veterinary technician, a supervisor who is an American Association for Laboratory Animal Science (AALAS) Laboratory Animal Technologist, eleven animal care technicians with various levels of AALAS technician certification, and two office personnel.
The HSC is registered with the US Department of Agriculture as a research facility and has maintained Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) accreditation since 1967. Animal care and use activities at West Virginia University are overseen by the Institutional Animal Care and Use Committee (IACUC). The Provost acts as the legal Institutional Official on behalf of the University.
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Mass Spectrometry Center
Operated by the Department of Biochemistry and Molecular Pharmacology in the School of Medicine, the Mass Spectrometry Center provides analysis and consultation services to biochemical and chemical investigators.
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Recombinant DNA Core Facility
Operated by the Department of Biochemistry and Molecular Pharmacology in the School of Medicine, the RDCF assists investigators in conducting molecular biology experiments. The following equipment and services are offered:
- Oligonucleotide synthesis
- Computer work-station/databank searches
- X-ray processor
- Videotapes of techniques in molecular cloning and recombinant DNA technology
- Large-scale isolation and purification of plasmid DNA
- Enzymes and purification kits
- DNA thermal cycler
- Radioanalytical imaging
- FPLC chromatography
- DNA sequencing
- Competent stocks of common E.Coli strains
- Training services for common techniques
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Transgenic Rodent Facility
The WVU School of Medicine was one of 41 U.S. medical schools to receive a prestigious four-year grant from the Howard Hughes Medical Institute for faculty recruitment in neurosciences and construction of a transgenic rodent facility.
This facility offers the following services:
- Assistance in the design of molecular constructs for the development of transgenic animals
- Generation of transgenic rats and mice containing those molecular constructs
- Assistance with detecting the transgenic animals among live-born pups
- Consultation in the rearing, analysis, and breeding of the animals
- Partial support maintenance and use of transgenic animal lines
- Implementation of transgenic technologies such as knockout, conditional gene deletion(Cre/loxP), and temporal transgene expression (e.g., a tetracycline-responsive promoter)
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