Overview
The Microscope Imaging Facility provides access to a wide range of imaging modalities for analysis of biological samples, including multiple systems for brightfield, epifluorescence, confocal, intravital and super resolution imaging. Three systems are equipped with environmental controls* for live cell imaging, and the multiphoton microscope includes anesthesia and physiological monitoring systems
for work in live animals. Two off-line image analysis workstations are available to the facility users for image processing and data analysis. The facility staff is dedicated to providing ongoing training and support to ensure the success of imaging projects.
Equipment
- Miltenyi UltraMicroscope II Light Sheet
- Olympus MVX10 Macroscope
- Nikon A1R Confocal/N-SIM-E*
- Olympus Slide Scanner
- Nikon A1RHD Multiphoton
- Zeiss 710 Confocal w/ Airyscan*
- Nikon Sweptfield Confocal*
- Zeiss Fluorescent
- Eppendorf Microinjection System
- Zeiss Tissue Culture
Contacts
Director
Neil Billington, PhD | neil.billington@hsc.wvu.edu | (304) 293-0942
Acknowledgements
Please remember to acknowledge the relevant grants that support the Microscope Imaging Facility in all your publications:
Imaging experiments were performed in the West Virginia University Microscope Imaging Facility which has been supported by the WVU Cancer Institute, the WVU HSC Office of Research and Graduate Education, and NIH grants P20GM121322 and P20GM144230.
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Nikon A1R/SIM: U54GM104942 & P20GM103434
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Nikon Multiphoton: S10OD026737
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Zeiss LSM710: P30GM103503 & P20GM103434
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Zeiss Fluorescent: P20GM103434
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Olympus VS120 Slide Scanner: P20GM103434
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Olympus MVX10: P20GM103434
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Zeiss Tissue Culture: P30GM103488 & P20GM103434
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Workstations 1 & 2: P20GM103434
Acknowledging the Microscope Imaging Facility
The Association of Biomolecular Resource Facilities (ABRF) has established recommended authorship guidelines that the Research Technology Support Facility recommends. Please see below for their recommended guidelines:
WHY CITE THE CONTRIBUTIONS OF A CORE OR CORE PERSONNEL?
- Core facility personnel are scientists. When they make a substantial intellectual and/or experimental contribution to a publication they deserve to be acknowledged just as any other co-author.
- Core facilities must charge for services provided according to cost accounting practices set up their home institution. Charging for services does not preclude authorship on manuscripts provided the Core laboratory individual has contributed to the research in a substantial way.
- The existence of core facilities depends in part on proper acknowledgment in publications. This is an important metric of the value of most core facilities. Proper acknowledgment of core facilities enables them to obtain financial and other support so that they may continue to provide their essential services in the best ways possible. It also helps core personnel to advance in their careers, adding to the overall health of the core facility.
Activities for which authorship are recommended:
- Author should make substantive contributions to the project
- Conception, design of project, critical input, or original ideas
- Acquisition of data, analysis and interpretation, beyond routine practices
- Draft the article or revise it critically for intellectual content
- Write a portion of the paper (not just materials and methods section)
- Intellectual contribution
- Final authority for the approval of article
- All contributors that do not meet the criteria of authorship should be recognized in the acknowledgements section, for example:
- Paid technical help
- Writing assistance
- Financial and material support
- Scientific advice
