Pathology/Histology Services

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The Anatomic Pathology Division of the Pathology Department offers various services, including the Autopsy Service, Surgical Pathology, Cytopathology and Neuropathology analysis. This group has a collection of paraffin embedded tissues that can be accessed for immunohistochemical analysis. A centralized Histology Laboratory, which provides routine processing, sectioning and staining of body tissues, as well as a wide variety of special stains and advanced immunohistochemical procedures are available on a charge basis. On-site technicians will section requested tissues and perform immunohistochemical labeling on a charge basis. Optionally, sections can be mounted on slides and the investigator may process immunohistochemical labeling. This section prepares 250,000 slides a year for pathologic examination. In the Cytopathology Laboratory, cellular morphology studies are performed on a variety of specimens, including gynecologic smears, body fluids and fine needle aspirations. The Cytopathology Laboratory examines 8,000 gynecologic specimens and 2,500 non-gynecologic specimens annually. About 300 fine needle aspirations are performed each year.

It is the goal of the Pathology Laboratory for Translational Medicine to provide the research community with the technical services necessary for growth and development in the world of investigative research. The services that will be provided by the laboratory are essential to an institution upholding a status of high recognition nationally and internationally. At a time when new discoveries generated by basic biomedical research are applied to pathology practice, accessibility to the most modern scientific methods becomes imperative. The following services will be provided at the Pathology Laboratory for Translational Medicine:

Routine Histology - Routine processing, cutting and H&E staining will be offered at our laboratory. Aided by automated equipment, our laboratory will provide this service in an appropriate time frame with consistent quality.

Immunohistochemistry - this diagnostic staining is done primarily on formalin-fixed, paraffin embedded tissues. This technique evaluates antigen expression and permits assessment of phenotypic properties, such as cell origin and prognostic features. With the aid of automated equipment the laboratory will provide the research community with expeditious, high quality service.

In Situ Hybridization - allows the localization of definitive nucleic acid sequences directly within a cell or tissue. High specificity is ensured through the action of complementary nucleic acid binding sequences. ISH techniques can be used to identify infectious agents in tissue sections, localize gene expression within individual cells, or detect specific DNA sequences in the genome of cells. We offer also FISH and CISH techniques.

Immunofluorescence Staining - is performed on frozen sections of skin, kidney, lymph node and tumors. It is used to diagnose immune-mediated diseases of the skin, mucosae and the kidney. It is also used to immunophenotype lymphoid proliferations and several other types of tumors. It allows the identification of specific cell-differentiation genes and the expression of reporter genes. Modified protocols may allow the study of paraffin sections.

Imaging – services will provide the research community with additional options and new opportunities to document photographically and quantify research material.

Interpretive - services will allow the research community to utilize the expertise of faculty members of The Department of Pathology. The anatomic pathologist’s morphologic diagnostic skills and expertise in correlating morphologic changes with biological phenomena to assist researchers in the study of their tissues.

Tissue Microarray - allows generation of multiple specimen slides that contain hundreds of individual tissues. Instead of incubating and analyzing samples one slide at a time, tissue microarrays (TMAs) allow you to examine hundreds of samples with just one slide. For example, an immunocytochemical analysis from 600 different tissues can be completed in a day.

TUNEL – Apoptosis plays an important role in the homeostasis and development of all tissues within an organism. In contrast to necrosis (cell death by accident), apoptosis is a well regulated physiological process. Any disturbance of the balance between cell proliferation and cell death maintained by apoptosis can result in serious disease, in particular cancer. TUNEL staining allows for interpretation of apoptotic cells. Charges applicable to TUNEL staining will involve any request for processing or microtomy. The TUNEL stain is $28.00 per slide.

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